Phylogenetic inference applied to germplasm bank characterization and interspecific breeding in passionfruit

Interspecific crosses between the more than 520 Passiflora species may or not be compatible. The sequences ITS, matK, psbA-trnH, rbcL and trnL-F were used to confirm identity estimate genetic distances among 48 accessions of a germplasm bank. Twenty for within distinct subgenera. phylogenetic resolution based on ITS data was superior that any single chloroplast marker, recommending it as DNA barcode this genus. However, tree topology Bayesian analysis using combination four markers possessed greater support subclades subgenus Passiflora, which contains interest breeders. We could identify clear cutoff value pairwise Kimura two-parameter (K2P) predict success failure crosses. Rather, clustering pairs together same closely related predicted probability wide cross compatibility reliably.